Anti-ANGPT2 Recombinant Antibody

Name: Anti-ANGPT2 Recombinant Antibody
SKU: TAB-769
Rating: 5 (1 reviews)

CAT#: TAB-769

Recombinant human monoclonal antibody expressed in CHO binding to human ANGPT2. Anti-ANGPT2 Therapeutic Antibody is a monoclonal antibody designed for the treatment of cancer.

Tested Data Gene Expression

Figure 1 Anti-ANGPT2 Therapeutic Antibody (TAB-769) in DB

Dot Blot analysis of TAB-769 was performed by coating with human ANGPT2 protein (His tag).

TAB-769 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Human IgG was used as a secondary antibody (1: 6000)

Figure 2 Anti-ANGPT2 Therapeutic Antibody (TAB-769) in WB

Western blot analysis of TAB-769 was performed with human ANGPT2 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-769 and HRP goat anti-Human IgG was used as a secondary antibody (1: 6000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)

ELISA

Figure 3 Anti-ANGPT2 Therapeutic Antibody (TAB-769) in ELISA

ELISA analysis of TAB-769 was performed by coating with human ANGPT2 protein (His tag). Then blocked with BSA and incubated with TAB-769. The HRP-conjugated goat anti-Human IgG was used as a secondary antibody (1: 6000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

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Normal Tissue Figure 1 Cerebral cortex

Neuronal cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11437/27034_B_8_5.jpg

Normal Tissue Figure 2 Colon

Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11437/27028_A_9_3.jpg

Normal Tissue Figure 3 Liver

Hepatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11437/27028_A_8_4.jpg

Normal Tissue Figure 4 Kidney

Cells in tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11437/27028_A_8_5.jpg

RNA Expression Figure 5 RNA cell line category: Group enriched (HMC-1, HSkMC, HUVEC TERT2, WM-115)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000091879-ANGPT2

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Specifications

Immunogen
The details of the immunogen for this antibody are not available.

Host Species
Human

Derivation
Human

Type
IgG1

Species Reactivity
Human

Applications
Suitable for use in ELISA, IP, FC, FuncS, Neut, IF, ICC and most other immunological methods.

MW
144.9 kDa

Related Disease
Solid tumors

Product Property

Purity
>95.0% as determined by analysis by RP-HPLC.

Storage
Store at -20°C for long-term storage. Store at 2-8°C for up to one month. Avoid freeze/thaw cycles.

Target

Alternative Names
ANGPT2;angiopoietin 2;angiopoietin-2;Ang2;ANG-2;Tie2-ligand;angiopoietin-2B;angiopoietin-2a;ANG2;AGPT2;

Gene ID
285

UniProt ID
O15123

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(Creative Biolabs Cat# TAB-769, RRID: AB_3112007)

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-769.pdf

MSDS

MSDS - TAB-769.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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TAB-0032CL-VHH	Anti-Human ANGPT2 VHH Single Domain Antibody	ELISA, Block, SPR	Single domain antibody
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CAT	Product Name	Application	Type
PFBL-397	Human Anti-ANGPT2 Recombinant Antibody (clone 5A12); Fab Fragment	WB, ELISA, FuncS	Human Fab
HPAB-AP234-YC-F(E)	Human Anti-ANGPT2 Recombinant Antibody (HPAB-AP234-YC-F(E)); Fab Fragment	Block, FuncS	Human Fab
HPAB-AP235-YC-F(E)	Human Anti-ANGPT2 Recombinant Antibody (HPAB-AP235-YC-F(E)); Fab Fragment	Block, FuncS	Human Fab
HPAB-AP236-YC-F(E)	Human Anti-ANGPT2 Recombinant Antibody (HPAB-AP236-YC-F(E)); Fab Fragment	Block, FuncS	Human Fab
HPAB-AP237-YC-F(E)	Human Anti-ANGPT2 Recombinant Antibody (HPAB-AP237-YC-F(E)); Fab Fragment	Block, FuncS	Human Fab

CAT	Product Name	Application	Type
TAB-077CT	Anti-Human ANG2 Recombinant Antibody (1A2)	ELISA, WB	Human antibody
TAB-0867CLV-F(E)	Human Anti-ANGPT2 Recombinant Antibody; Fab Fragment (TAB-0867CLV-F(E))	ELISA	Human Fab
TAB-0869CLV-F(E)	Human Anti-ANGPT2 Recombinant Antibody; Fab Fragment (TAB-0869CLV-F(E))	SPR, Inhib, FuncS	Human Fab
TAB-0870CLV-F(E)	Human Anti-ANGPT2 Recombinant Antibody; Fab Fragment (TAB-0870CLV-F(E))	FuncS	Human Fab
TAB-0871CLV-F(E)	Human Anti-ANGPT2 Recombinant Antibody; Fab Fragment (TAB-0871CLV-F(E))	SPR, Inhib, FuncS	Human Fab

CAT	Product Name	Application	Type
TAB-0886CLV-F(E)	Human Anti-ANGPT2 Recombinant Antibody; Fab Fragment (TAB-0886CLV-F(E))	ELISA, WB	Humanized Fab
TAB-0887CLV-F(E)	Human Anti-ANGPT2 Recombinant Antibody; Fab Fragment (TAB-0887CLV-F(E))	ELISA, WB	Humanized Fab
TAB-0888CLV-F(E)	Human Anti-ANGPT2 Recombinant Antibody; Fab Fragment (TAB-0888CLV-F(E))	ELISA, WB	Humanized Fab
TAB-0889CLV-F(E)	Human Anti-ANGPT2 Recombinant Antibody; Fab Fragment (TAB-0889CLV-F(E))	ELISA, WB	Humanized Fab
TAB-0890CLV-F(E)	Human Anti-ANGPT2 Recombinant Antibody; Fab Fragment (TAB-0890CLV-F(E))	ELISA, WB	Humanized Fab

CAT	Product Name	Application	Type
TAB-0894CLV-F(E)	Mouse Anti-ANGPT2 Recombinant Antibody; Fab Fragment (TAB-0894CLV-F(E))	ELISA, WB	Mouse Fab
TAB-0895CLV-F(E)	Mouse Anti-ANGPT2 Recombinant Antibody; Fab Fragment (TAB-0895CLV-F(E))	ELISA, WB	Mouse Fab
TAB-0896CLV-F(E)	Mouse Anti-ANGPT2 Recombinant Antibody; Fab Fragment (TAB-0896CLV-F(E))	ELISA, WB	Mouse Fab
TAB-0897CLV-F(E)	Mouse Anti-ANGPT2 Recombinant Antibody; Fab Fragment (TAB-0897CLV-F(E))	ELISA, WB	Mouse Fab
TAB-0894CLV-S(P)	Mouse Anti-ANGPT2 Recombinant Antibody; scFv Fragment (TAB-0894CLV-S(P))	ELISA, WB	Mouse scFv

CAT	Product Name	Application	Type
MOR-0170	Hi-Affi™ Rabbit Anti-ANGPT2 Recombinant Antibody (clone DS170AB)	IHC-Fr, WB	Rabbit IgG

CAT	Product Name	Application	Type
MRO-0094-CN	Rabbit Anti-ANGPT2 Recombinant Antibody (clone JM71-34)	WB, IHC	Rabbit IgG
HPAB-AP235-YC	Human Anti-ANGPT2 Recombinant Antibody (HPAB-AP235-YC)	Block, FuncS	Human IgG
HPAB-AP236-YC	Human Anti-ANGPT2 Recombinant Antibody (HPAB-AP236-YC)	Block, FuncS	Human IgG
HPAB-AP237-YC	Human Anti-ANGPT2 Recombinant Antibody (HPAB-AP237-YC)	Block, FuncS	Human IgG
HPAB-AP238-YC	Human Anti-ANGPT2 Recombinant Antibody (HPAB-AP238-YC)	Block, FuncS	Human IgG

CAT	Product Name	Application	Type
AFC-TAB-461CQ	Afuco™ Anti-ANGPT2 Recombinant Antibody (AFC-TAB-461CQ), ADCC Enhanced	ELISA, IHC, FC, IP, IF, Inhib	Human IgG1, κ

CAT	Product Name	Application	Type
HPAB-AP234-YC-S(P)	Human Anti-ANGPT2 Recombinant Antibody (HPAB-AP234-YC-S(P)); scFv Fragment	Block, FuncS	Human scFv
HPAB-AP235-YC-S(P)	Human Anti-ANGPT2 Recombinant Antibody (HPAB-AP235-YC-S(P)); scFv Fragment	Block, FuncS	Human scFv
HPAB-AP236-YC-S(P)	Human Anti-ANGPT2 Recombinant Antibody (HPAB-AP236-YC-S(P)); scFv Fragment	Block, FuncS	Human scFv
HPAB-AP237-YC-S(P)	Human Anti-ANGPT2 Recombinant Antibody (HPAB-AP237-YC-S(P)); scFv Fragment	Block, FuncS	Human scFv
HPAB-AP238-YC-S(P)	Human Anti-ANGPT2 Recombinant Antibody (HPAB-AP238-YC-S(P)); scFv Fragment	Block, FuncS	Human scFv

CAT	Product Name	Application	Type
VS-0424-XY12	AbPlus™ Anti-ANGPT2 Magnetic Beads (VS-0424-XY12)	IP, Protein Purification
VS-0724-YC1209	AbPlus™ Anti-ANGPT2 Magnetic Beads (VS-0724-YC1209)	IP, Protein Purification

CAT	Product Name	Application	Type
VS-0325-FY79	Human Anti-ANGPT2 (clone MEDI1/5) scFv-Fc Chimera	ELISA, Inhib, IF	Human IgG1, scFv-Fc

CAT	Product Name	Application
VS-0525-XY367	Anti-ANGPT2 Immunohistochemistry Kit	IHC
VS-0525-XY368	Anti-Mouse ANGPT2 Immunohistochemistry Kit	IHC
VS-0525-XY369	Anti-Rat ANGPT2 Immunohistochemistry Kit	IHC

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